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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cell. 2018 Jul 26;174(5):1082–1094.e12. doi: 10.1016/j.cell.2018.06.040

Figure 5. Role of microtubule-mediated transport in selective retrotransposition.

Figure 5.

(A and B) γ-H2Av staining to probe the amount of DNA damage in oocyte upon Ago3&Aub depletion (A) or I-element invasion (B). Arrowhead points the oocyte that possesses DNA breaks. Depolymerizing microtubule (M.T.) by Demecolcine-feeding alleviates the DNA damage in oocyte. Data quantification is presented in Figure S6A and S6B.

(C) RNA-FISH to detect HMS-Beagle or 3S18 transcripts upon microtubule depolymerization.

(D) FISH signal quantification to test the potential contribution of early oocyte transcription on the signal detected in the oocytes of stage 8 egg chambers. FISH signal in the oocytes of stage 2 egg chambers defines the maximum amount of mRNAs generated from early stage oocyte. Since there is no oocyte transcription from stage 2 to stage 8. More than 97% of mRNAs in the oocytes of stage 8 egg chambers are transported from nurse cells. Data are represented as mean ± SD. n = 6 ovarioles from 3 animals.