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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cell. 2018 Jul 26;174(5):1082–1094.e12. doi: 10.1016/j.cell.2018.06.040

Figure 6. Retrotransposons rarely mobilize in nurse cells.

Figure 6.

(A) FACS sorting to purify the nuclei of nurse cells for DNA sequencing. In Drosophila ovaries, the genome of somatic cells (most of them are follicle cells) can only reach 32C. In contrast, nurse cells can achieve 1024C via endo-replication. We have purified the nuclei of cells that have ⩾ 64C DNA.

(B) Upon depleting Ago3&Aub, the number of potential new insertions detected from the DNA of sorted nurse cell. Without the protection from piRNAs, few new insertions were detected in nurse cells.

(C) Either upon depleting Ago3&Aub (left panel) or I-element-invasion (right panel), the number of potential new insertions detected when the transposon mRNAs are restricted within nurse cells (by blocking microtubule-mediated transport). Transposons barely target nurse cell for mobilization.