Figure 6.

Adenosine receptors gene expression assayed by quantitative real-time RT-PCR in C6 cells. After total RNA isolation, A_2A (panel a), A_2B (panel b), and A₃ (panel c) receptor gene expression levels were detected by TaqMan universal PCR following the protocol indicated in “Materials and Methods”. β-actin was used as an endogenous control in all assays. Treatments were performed for 30 min with 50 µM hydrogen peroxide (H₂O₂) and DB, NAB, and LB extracts in C6 cells. (d) Heatmap of gene expression levels obtained in the analyzed samples. Data are means ± SEM of, at least, three independent assays; * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from their corresponding control (C) or considered bars according to Student’s t test; # p < 0.05 significantly different from control (C) according to one-way ANOVA test.