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. 2019 Jun 3;11(6):1258. doi: 10.3390/nu11061258

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Adenosine receptors gene expression assayed by quantitative real-time RT-PCR in SH-SY5Y cells. After total RNA isolation, A₁ (panels a and b), A_2A (panels c and d), and A_2B (panels e and f) gene expression levels were detected by TaqMan universal PCR following the protocol indicated in “Materials and Methods”. β-actin was used as an endogenous control in all assays. Treatments were performed for 30 min (a, c, e) and 24 h (b, d, f) with 50 µM hydrogen peroxide (H₂O₂) and DB, NAB, and LB extracts in SH-SY5Y cells. (g) Heatmap of gene expression levels obtained in the analyzed samples. Data are means ± SEM of, at least, three independent assays; # p < 0.05, ## p < 0.01, and ### p < 0.001, significantly different from control (C) or considered bars according to one-way ANOVA test.

Adenosine receptors gene expression assayed by quantitative real-time RT-PCR in SH-SY5Y cells. After total RNA isolation, A₁ (panels a and b), A_2A (panels c and d), and A_2B (panels e and f) gene expression levels were detected by TaqMan universal PCR following the protocol indicated in “Materials and Methods”. β-actin was used as an endogenous control in all assays. Treatments were performed for 30 min (a, c, e) and 24 h (b, d, f) with 50 µM hydrogen peroxide (H₂O₂) and DB, NAB, and LB extracts in SH-SY5Y cells. (g) Heatmap of gene expression levels obtained in the analyzed samples. Data are means ± SEM of, at least, three independent assays; # p < 0.05, ## p < 0.01, and ### p < 0.001, significantly different from control (C) or considered bars according to one-way ANOVA test.