Table 2.
Primer sequences and PCR conditions for the investigated genes.
| Gene | Accession Number | Forward Primer (5′–3′) | Reverse Primer (3’–5’) | Product Length (bp) | Annealing Temperature (°C) | Cycles. (N°) |
|---|---|---|---|---|---|---|
| Aggrecan | NM_001135.3 | CTTGGACTTGGGCAAACTGC | CACTAAAGTCAGGCAGGCCA | 143 | 60 | 35 |
| Collagen II | NM_001844.4 | TGGATGCCACACTCAAGTCC | GCTGCTCCACCAGTTCTTCT | 254 | 60 | 35 |
| Collagen X | NM_000493.3 | AAACCTGGACAACAGGGACC | CGACCAGGAGCACCATATCC | 124 | 60 | 35 |
| SOX9 | NM_000346.3 | GAAGGACCACCCGGATTACA | GCCTTGAAGATGGCGTTGG | 120 | 60 | 35 |
| GAPDH | NM_002046.4 | GTCAGTGGTGGACCTGACCT | AGGGGTCTACATGGCAACTG | 420 | 56 | 30 |