Figure 4.

Effects of compounds on cell viability in colorectal cancer cells. Human colorectal cancer HCT116 and DLD-1 cells were cultured and treated with 10-fold diluted (10 or 100 μg/mL) extracts (A) XS-(fr)-M, (B) XS-(fr)-C, (C) TT-(fr)-A, (D) TT-(fr)-M in cultured medium (DMEM) or autophagy-inducing medium Earle’s Balanced Salt Solution (EBSS) for 24 h. The CellTiter-Glo reagent was added to cells, followed by measurement of the cellular ATP level to reflect cell viability. The results are expressed as the mean ± SEM from three individual experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 for cells in starvation medium EBSS vs. cells with the same treatment in regular medium (Control).