Table 1.
RTX toxin concentrations, lipopolysaccharide (LPS) contaminations and total amounts of the proteins obtained from one liter of culture.
| RTX Toxin 1 | Without Detergent Wash | 1% Triton X-100 Wash | 1% Triton X-114 Wash | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Conc. 2 (mg/mL) |
LPS 3 (EU/mg) |
Total 4 (mg) |
Conc. 2 (mg/mL) |
LPS 3 (EU/mg) |
Total 4 (mg) |
Conc. 2 (mg/mL) |
LPS 3 (EU/mg) |
Total 4 (mg) |
|
| CyaA | 1.3 | >4 × 105 | 6 | 1.0 | 12 | 6 | 1.2 | 8 | 6 |
| RtxA | 2.0 | >1 × 106 | 8 | 2.1 | 115 | 8 | 2.0 | 25 | 8 |
| HlyA | 1.4 | >1 × 106 | 4 | 1.3 | 95 | 4 | 1.4 | 15 | 4 |
| ApxIA | 1.9 | >1 × 106 | 8 | 2.1 | 85 | 8 | 1.6 | 15 | 8 |
1 adenylate cyclase toxin-hemolysin (CyaA), RtxA, α-hemolysin (HlyA) and ApxIA toxins were purified as described in detail in Materials and Methods. 2 Protein concentration was determined by the Bradford assay. 3 LPS content was determined by the Chromogenic LAL assay. 4 Total protein amount.