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. 2019 Jun 14;11(6):339. doi: 10.3390/toxins11060339

Figure 7.

Figure 7

Toxicity and TNA of bacterial culture supernatants. Bacterial culture supernatants grown in (A) BHI or (B) TSB media incubated with indicated polyclonal antibodies to measure relative cytotoxicity and neutralization post a 3 h incubation in PMN cells. Error bars indicate measurements made in triplicates. (C) Toxicity profiles of SF8300 wild-type and LukAB-deficient isogenic mutant grown in BHI or TSB media on differentiated THP-1 cells measured 4 h post incubation. Error bars represent measurements made in triplicates. (D) Measurement of toxicity of purified toxoids such as α-toxin and BCPFTs on differentiated THP-1 cells. CTG readout was carried out 1 h post incubation with toxoids and error bars indicate measurements made in duplicates. (E) TNA results of supernatants from SF8300WT or SF8300ΔlukAB strains grown in TSB and BHI media and incubated with αLukAB, αLukS, αLukF, or αHla, or a combination as labeled for 4 h to measure extent of neutralization indicated by cell viability measurements. Error bars represent measurements made in duplicates.