Table 3.
The ratio of absorbance at 490 nm to absorbance at 750 nm (A490/A750).
Tested Sample | Concentration (µL mL−1) | A490/A750 | |||
---|---|---|---|---|---|
N. fischeri | F. oxysporum | Botrytis sp. | P. setifera | ||
Crude extract | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 0.97 | 1.05 | 1.09 | 0.91 | |
50 | 1.00 | 1.07 | 1.10 | 0.80 | |
100 | 0.99 | 1.07 | 1.08 | 0.91 | |
500 | 1.04 | 1.15 | 1.14 | 0.74 | |
Purified extract | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 1.02 | 1.07 | 1.06 | 1.07 | |
50 | 0.99 | 1.03 | 1.06 | 0.92 | |
100 | 0.98 | 1.01 | 1.11 | 0.71 | |
500 | 0.95 | 1.01 | 1.03 | 0.52 | |
Polar fraction of the extract | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 0.96 | 1.02 | 1.02 | 1.01 | |
50 | 1.00 | 1.06 | 1.03 | 0.94 | |
100 | 0.99 | 1.05 | 1.03 | 0.90 | |
500 | 1.04 | 1.10 | 1.04 | 0.94 | |
Fraction 1 | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 0.96 | 1.00 | 0.98 | 0.67 | |
50 | 0.98 | 1.02 | 1.01 | 0.59 | |
100 | 0.97 | 1.01 | 1.02 | 0.75 | |
500 | 0.94 | 0.97 | 0.95 | 0.59 | |
Fraction 4 | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 0.95 | 0.97 | 1.04 | 0.67 | |
50 | 0.93 | 1.04 | 0.97 | 0.78 | |
100 | 0.98 | 1.13 | 1.01 | 0.96 | |
500 | 1.03 | 1.44 | 1.20 | 1.26 | |
Fraction 5 | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 0.93 | 0.95 | 0.95 | 0.58 | |
50 | 0.93 | 0.94 | 0.93 | 0.62 | |
100 | 0.93 | 0.95 | 0.94 | 0.75 | |
500 | 1.01 | 1.02 | 1.02 | 0.89 | |
Fraction 6 | 0 | 0.93 | 0.96 | 0.89 | 0.89 |
5 | 1.01 | 1.07 | 1.09 | 0.69 | |
50 | 1.04 | 1.13 | 1.11 | 0.85 | |
100 | 1.02 | 1.12 | 1.05 | 0.79 | |
500 | 1.00 | 1.06 | 1.10 | 0.82 |
—A490/A750 < 0.75,
—0.75 ≤ A490/A750 ≤ 0.95,
—0.95 ≤ A490/A750 < 1.05,
—1.05 ≤ A490/A750 ≤ 1.10,
—1.10 < A490/A750 ≤ 1.20,
—1.20 < A490/A750.