Table 2.

Sequences of the complementarity determining regions (CDRs) of different affinity matured mycolactone specific antibodies identified by yeast display after error prone PCR. CI represents the confidence interval.

Clone ID	HCDR1	HCDR2	HCDR3	Percentage Sequence Abundance			Yeast-Based Affinity	CI
				Selection Output	2 rds Affinity Maturation	4 rds Affinity Maturation
parental	GGTFSSYA	IIPIFGTA	ARVRWEPQSGYYHGMDVW	91%	17%		470 nM	241–720
AM_1	GGTFSSYA	IIPIFGTA	ARVRWEPRSGYYHGMDVW		10%		360 nM	260–450
AM_2	GGTFSSYA	IIPIFGTA	ARVRWVPRSGYYHGMDVW		7%		345 nM	181–676
AM_3/M3_A10	GGTFSSYA	IIPIFGTA	ARVRWVPQSGYYHGMDVW		10%	20%	149 nM	71–271
AM_4	GGAFSRYA	IIPIFGTA	ARVRWVPQSGYYHGMDVW		29%	54%	145 nM	69–298
AM_5	GGTFSRYA	IVPIFGTA	ARVRWVPQSGYYHGMDVW		24%	16%	212 nM	159–324