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. 2019 Jul 15;12:181. doi: 10.1186/s13068-019-1525-5

Fig. 2.

Fig. 2

Allelic series of mutations in LAC8 gene (Bradi2g23370) identified by TILLING and peptide alignment of different Brachypodium laccase C-terminals. a Features of the mutagenized lines identified by TILLING. Lines harboring mutation in non-translated region (intron, 5′ or 3′UTR) were discarded from the table. b Alignment of amino acids present in the C-terminal region of all Brachypodium laccases present in the genome of Brachypodium distachyon Bd21-3 accession. The codon encoding tryptophan at position 551 is replaced by a stop codon in the mutant line Bd5731. Colors illustrate the conserved regions between proteins