IL2RA genotype associations on differentiation of CD4+ T cells. Homozygous carriers of both MS-associated IL2RA SNPs, rs2104286 and rs11256593, are defined by TT for the risk genotype (N = 23) and CC for the protective genotype (N = 23). Gating strategy is outlined in Figure 1A–E. (A) Differences between TT carriers and CC carriers on frequency of CD127+CD25+ naïve (Paired t test, mean of difference = −4.6; 95% CI = −8.5 to −0.7), T-CM (Paired t test, mean of difference = −5.6; 95% CI = −10.4 to −0.9), CM (Paired t test, mean of difference = 0.6; 95% CI = −2.7 to 3.9), EM (Paired t test, mean of difference = 1.2; 95% CI = −4.0 to 6.4) and L-EM cells (Paired t test, mean of difference = 5.1; 95% CI = −3.6 to 13.8). (B) Differences between TT carriers (N = 23) and CC carriers (N = 23) on surface expression (MFI) of CD25 on CD127+CD25+ naïve (Paired t test, mean of difference = −16.9; 95% CI = −56.5 to 22.8), T-CM (Paired t test, mean of difference = 63.9; 95% CI = 13 to 114.8), CM (Paired t test, mean of difference = 61.5; 95% CI = 5.6 to 117.4), EM (Paired t test, mean of difference = 120; 95% CI = 13.4 to 226.7) and L-EM cells (Paired t test, mean of difference = 153.9; 95% CI = 0.9 to 306.8).