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. 2019 Jun 25;8(6):634. doi: 10.3390/cells8060634

Figure 3.

Figure 3

IL2RA genotype associations on recent thymic emigrants and CD31−CD45RA+ CD4+ T cells. (A) Gating of CD127+ cells on CD4+ T cells by use of matched isotype control. (B) Matched isotype control for CD25 and CD127 on CD4+ T cells. (C) Gating of recent thymic emigrants (RTE; CD31+CD45RA+) and CD31−CD45RA+ cells. RTE and CD31−CD45RA+ cells are gated from CD127+CD4+ T cells to exclude contamination from TReg cells. (D) Gating of CD25+ cells on CD4+ T cells by use of matched isotype control. (E) Matched isotype control for CD25. (F) Frequency of recent thymic emigrant (RTE; CD31+CD45RA+) cells and CD31−CD45RA+ cells in relation to CD4+ T cells. (G,I) Homozygous carriers of both MS-associated IL2RA SNPs rs2104286 and rs11256593 are defined by TT for the risk genotype and CC for the protective genotype. Differences between TT carriers (N = 16) and CC carriers (N = 16) on frequency of CD25+ cells in RTE (Paired t test, mean of difference = −8.0; 95% CI = −13.5 to −2.6) and CD31−CD45RA+ (Paired t test, mean of difference = −4.3; 95% CI = −11.4 to 2.7) T cells. (H,J) Differences between TT carriers (N = 16) and CC carriers (N = 16) on surface expression (MFI) of CD25 in CD25+RTE (Paired t test, mean of difference = −17.8; 95% CI = −67.1 to 31.6) and CD25+CD31−CD45RA+ (Paired t test, mean of difference = 120.3; 95% CI = 29.3 to 211.3) cells.