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. 2019 Jun 25;8(6):634. doi: 10.3390/cells8060634

Figure 4.

Figure 4

IL2RA genotype associations on CD4+ T follicular helper subsets. (A) Gating of CD127+ cells on CD4+ T cells by use of matched isotype control. (B) Gating of CXCR5− cells, defined as non-follicular T helper (CXCR5− TH) cells and T follicular helper (TFH) cells, defined by being PD-1+CXCR5+. Gating are performed on CD127+ CD4+ T cells to exclude contamination from TReg cells and by use of matched isotype control. (C) Matched isotype control for CXCR5 and PD-1 on CD4+ T cells. (D) On CXCR5− TH cells, differential expression of CXCR3 and CCR6 defines three subsets: TH1 (CXCR3+CCR6−), Th17.1 (CXCR3+CCR6+) and TH17 (CXCR3−CCR6+). Gating are performed by use matched isotype control. (E) On TFH cells, differential expression of CXCR3 and CCR6 defines tree subsets: TFH1 (CXCR3+CCR6−), TFH2 (CXCR3−CCR6−) and TFH17 (CXCR3−CCR6+ TFH). Gating are performed by use matched isotype control. (F) Matched isotype control for CCR6 and CXCR3 on CD4+ T cells. (G,H) Homozygous carriers of both MS-associated IL2RA SNPs rs2104286 and rs11256593 are defined by TT for the risk genotype (N = 25) and CC for the protective genotype (N = 25). Difference between TT and CC carriers on the frequency of CD25+ cells in TFH cells (Paired t test, mean of difference = 3.7; 95% CI = 0.7 to 6.7) and the surface expression (MFI) of CD25 on CD25+ TFH cells (Paired t test, mean of difference = 20.7; 95% CI = −20 to 61.4). (I) Frequency of TFH1 (CXCR3+CCR6− TFH), TFH2 (CXCR3−CCR6− TFH) and TFH17 (CXCR3−CCR6+ TFH) cells in relation to CD4+ T cells. (J,K) Difference between carriers of the IL2RA risk genotype TT (N = 25) and the protective genotype CC (N = 25) on the frequency of CD25+ cells in TFH1 (Paired t test, mean of difference = 5.6; 95% CI = 2.4 to 8.7), TFH2 (Paired t test, mean of difference = 0.1; 95% CI = −3.2 to 3.4) and TFH17 (Paired t test, mean of difference = −1; 95% CI = −4.8 to 2.9) cells and the surface expression (MFI) of CD25 on CD25+TFH1 (Paired t test, mean of difference = 23.1; 95% CI = −20.6 to 66.8), CD25+TFH2 (Paired t test, mean of difference = 20.6; 95% CI = −22.5 to 63.8), and CD25+TFH17 (Paired t test, mean of difference = 63; 95% CI = −10.2 to 136.2) cells.