Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 6;28(4):413–424. doi: 10.1177/0963689719827943

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 4. — Secretion of KTEL-deleted codon-optimized CDNF is stable in clones cultured on Petri dish. (a) The secretion of KTEL-deleted codon-optimized CDNF was stable for 14 days in non-subcultivated cell cultures. In contrast, the secretion of wtCDNF decreased during the time course in cell cultures, which were not subcultivated during the experiment (p<0.001, repeated measures ANOVA with Bonferroni correction post hoc). (b) Secreted portion of CDNF during 1 h incubation from the total expressed CDNF (CDNF concentration in the medium + cell lysate) was similar in case of subcultivated and non-subcultivated clones expressing KTEL-deleted codon-optimized CDNF. Instead, the secreted portion of wtCDNF increased in the subcultivated cells (p=0.005, paired samples t-test). Data are presented as mean ± SEM. *** p<0.001, ** p<0.01.