Table 1.
Endogenous CDNF and MANF Protein Concentrations in Cell Lysates and Conditioned Media of Selected Cell Lines of Human Origin. Results are Shown as Average ± SEM (n=3).
| Human cell line | ARPE-19 | HeLa | EA.hy926 | SH-SY5Y | U-87 MG |
|---|---|---|---|---|---|
| MANF | |||||
| Conditioned medium (ng/ml) | 0.41 ± 0.03 | 3.99 ± 0.07 | 2.15 ± 0.16 | 1.27 ± 0.08 | 4.20 ± 0.46 |
| Cell lysate (ng/ml) | 91.7 ± 2.1 | 128.3 ± 2.4 | 57.2 ± 2.7 | 85.3 ± 1.1 | 70.5 ± 2.2 |
| % secreted | 0.4 | 3.0 | 3.6 | 1.5 | 5.6 |
| CDNF (ng/ml) | |||||
| Conditioned medium (ng/ml) | n.d. | n.d. | n.d. | n.d. | n.d. |
| Cell lysate (ng/ml) | 0.49 ± 0.02 | 0.73 ± 0.03 | 0.36 ± 0.01 | 0.37 ± 0.01 | 0.43 ± 0.02 |
| % secreted | n.a. | n.a. | n.a. | n.a. | n.a. |
| MANF vs. CDNF in cell lysate (times) | 187 | 176 | 159 | 231 | 164 |
n.d. = not detected, n.a. = not applicable.