Skip to main content
. 2019 Jun 19;20(12):2986. doi: 10.3390/ijms20122986

Figure 1.

Figure 1

Comparison of the expression of selected proteins in cytosolic (CYT) and high-purity mitochondrial (MIT) fractions isolated from MCF7 cells using a QProteome Mitochondria Isolation Kit (see Section 4). The level of tested proteins was assessed using western blot analysis employing relevant antibodies (see Section 4). Smac/Diablo (Second mitochondria-derived activator of caspases/Direct IAP binding protein with low pI, mitochondrial intermembrane space), SDHA (succinate dehydrogenase complex flavoprotein subunit α, inner mitochondrial membrane), CPS1 (carbamoyl-phosphate synthetase 1, mitochondrial matrix), calnexin (endoplasmic reticulum), β-actin (representative cytoskoletal protein), GAPDH (glyceraldehyde 3-phosphate dehydrogenase, cytosol), Hop (Hsp70-Hsp90 organizing protein, cytosol).