Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 19;20(12):2986. doi: 10.3390/ijms20122986

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Confirmation of changes in protein expression detected by 2D analysis employing western blot analysis. (a) Western blot was performed with whole-cell lysates of paclitaxel-sensitive MCF7 cells and paclitaxel-resistant MCF7/PacR cells. Levels of tested proteins were assessed using western blot analysis employing relevant antibodies (see Section 4). β-actin served as a loading control. Representative results are from three independent experiments. (b) Densitometric analysis of western blots normalized to β-actin level. The expression of proteins in MCF7/PacR was compared to the expression in MCF cells (100%), and relative values were normalized to β-actin levels. Columns represent mean values of band volume ± SEM from three experimental values. *** p < 0.001 compared to the volume in MCF7 cells. NS = statistically non-significant difference.