Figure 6.

Expression of CPS1 (carbamoyl-phosphate synthetase 1) in paclitaxel-sensitive MCF7 cells and paclitaxel-resistant MCF7/PacR cells. The expression was assessed employing FACS (see Section 4). The data shown were obtained in one representative experiment from three independent experiments. (a) Histograms of MCF7 and MCF7/PacR cells, which were stained with a secondary antibody (black) or stained with a specific CPS1 antibody and then with the secondary antibody (red). (b) The number of CPS1 positive cells vs. negative cells (ratio) in MCF7 and MCF7/PacR cell population. Columns represent the mean value of the ratio ± SEM from two experimental values. * p < 0.05 compared to the ratio in paclitaxel-sensitive MCF7 cells.