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. 2019 Jun 24;116(28):13970–13976. doi: 10.1073/pnas.1820001116

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Copyright © 2019 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 5. — GcoA-F169A converts syringol in vivo. (A) A pathway for in vivo syringol O-demethylation to pyrogallol and cleavage to 2-pyrone 6-carboxylate is proposed. (B) After 6 h, strains were analyzed for their ability to turn over syringol via ¹H NMR spectroscopy. Syringol (green) is completely converted to pyrogallol (pink), 3MC (blue), or 2-pyrone 6-carboxylate (purple) in AM157. The WT GcoA enzyme in AM156 shows only small amounts of conversion. AM155, which does not express GcoA, shows no conversion.