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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Biomaterials. 2019 Mar 21;206:13–24. doi: 10.1016/j.biomaterials.2019.03.026

Fig. 3. Glutathione-triggered release of peptide cargo from LProd.

Fig. 3.

(A) TEM image of LProd before and after a 4-h reduction by 10 mM GSH. (B) Distributions of hydrodynamic diameter of LProd (the presence of GSH or not) measured by DLS. (C) LProd was incubated in PBS solution (pH 7.4), and no released peptide was detected in the supernatant (10000g centrifugation) after a 4-h incubation. LProd was incubated in intracellular-redox milieu-mimic PBS solution (10 mM GSH, pH7.4), and released peptide peak was detected in the supernatant (10000g centrifugation) after a 4-h incubation. A 22-mer peptide (Seq.: EIYIAQELRRIGDEFN-AYYA) was used as internal reference, termed Ctrl, and its retention time was about 17 min. The peak 1 is the PMI checked by ESI-MASS. (D) PMI-Cys release from LProd in intracellular redox environment mimic solution (including 10 mM GSH) or GSH-free solution quantified by HPLC.