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. 2019 Jun 24;116(28):14154–14163. doi: 10.1073/pnas.1813580116

Fig. 1.

Fig. 1.

ER stress induced by C321R-LAMB2 disparately regulates the UPR branches in podocytes at the early stage of NS. Primary podocytes (P1 or P2) were isolated and cultured from Tg-WT, Tg-C321R, and Lamb2+/− mice at P27. (A) Representative immunoblots of p-IRE1α, IRE1α, and XBP1s expression in primary podocyte lysates of the indicated genotypes. A densitometry analysis of p-IRE1α normalized to IRE1α and XBP1s normalized to β-actin was performed in the podocyte lysates. (B) Representative immunoblots of p-eIF2α, eIF2α, and ATF4 expression in primary podocytes of the indicated genotypes. A densitometry analysis of p-eIF2α normalized to eIF2α and ATF4 normalized to β-actin was conducted in the podocyte lysates. (C) Representative immunoblot of p50ATF6 expression in primary podocytes of the indicated genotypes. A densitometry analysis of p50ATF6 normalized to β-actin was performed in the podocyte lysates. Quantification data represent the mean ± SD of 5 independent experiments. *P < 0.05; **P < 0.01. NS, not significant by ANOVA.