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. 2019 Jun 24;116(28):14055–14064. doi: 10.1073/pnas.1816981116

Fig. 4.

Fig. 4.

Reduced dTcf/Pan promotes the formation of tumors with different characteristics in a context-dependent manner. (A and B) Confocal micrographs showing the polarity marker Discs large (Dlg, red and gray) in tumorous discs of the following genotypes: ap-Gal4, UAS-GFP, UAS-Yki, UAS-dTcf/PanRNAi (A); and ap-Gal4, UAS-GFP, UAS-EGFR, UAS-dTcf/PanRNAi (B). XZ and YZ cross-sections are shown in the bottom and right-hand side, respectively. (C and D) Confocal micrographs showing matrix metalloprotease 1 (Mmp1, red and gray) in tumorous discs of the following genotypes: ap-Gal4, UAS-GFP, UAS-Yki, UAS-dTcf/PanRNAi (C); and ap-Gal4, UAS-GFP, UAS-EGFR, UAS-dTcf/PanRNAi (D). (E) Confocal micrograph of an ap-Gal4, UAS-GFP third-instar wing imaginal disk showing the expression of Twist (Twi, red). (F and G) Confocal micrographs showing the Twist protein (red and gray) in tumorous discs of the following genotypes: ap-Gal4, UAS-GFP, UAS-Yki, UAS-dTcf/PanRNAi (F); and ap-Gal4, UAS-GFP, UAS-EGFR, UAS-dTcf/PanRNAi (G). GFP is shown in green, and green and gray in G. DAPI labels the nuclei and is shown in blue (Scale bars: 100 μm).