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. 2019 Jun 24;116(28):14174–14180. doi: 10.1073/pnas.1821194116

Fig. 4.

Fig. 4.

Rad51 is a direct YAP/TAZ-TEAD target gene. (A) TEAD4 binding signals from ENCODE were analyzed in ChIP-seq datasets from h1-hESCs (human embryonic stem cells), HCT116 (colon cancer), Ishikawa (endometrial adenocarcinoma), and SK-N-SH (neuroblastoma) cells in the promoter region of the Rad51 gene. (B) Binding of TEAD4 on the Rad51 promoter was analyzed using ChIP in MDA-MB-231 (Left) and Hs578t (Right) cells. (C) MDA-MB-231 cells expressing dominant-negative TEAD4 were transfected with pRad51-Luc that utilizes the Rad51 promoter to control expression of firefly luciferase and assayed for Rad51 transcriptional activity. (D) Rad51 abundance was quantified by immunoblotting in MDA-MB-231 cells expressing dominant-negative TEAD4. Densitometry was assessed using ImageJ (Right bar graph). Dot plots (mean ± SD) represent 3 independent experiments. *P ≤ 0.05, ***P ≤ 0.0005 by 2-tailed t test.