Fig. 2.

Depletion of phagocytic cells influences mosquito survival after bacterial challenge. Mosquitoes were treated with either control or CLDs, and then subjected to injury (sterile PBS injection) or bacterial challenge. Survivorship was monitored in mosquitoes every day for 10 d to evaluate the effects of injury (A), S. marcescens (B), or S. aureus (C) challenge. Phagocyte depletion (CLD) in mosquitoes results in a high susceptibility to bacterial infections. Error bars represent the mean ± SEM of 3 independent replicates. In each replicate, 30 female mosquitoes were used for each experimental treatment. Data were analyzed by a log-rank (Mantel-Cox) test using GraphPad Prism 6.0.