Fig. 1.
CpdX and CpdX-D3 and their respective enantiomers are as efficient as Dex at decreasing skin inflammations generated in atopic dermatitis-like and psoriasis-like models, and in a TPA-induced irritant contact dermatitis. (A) Experimental protocol for an MC903-induced atopic dermatitis-like skin inflammation. (B) The qRT-PCR for RNA transcripts of proinflammatory genes in BALB/c mouse ears treated as indicated. The statistical significance compared with the MC903 treatment was calculated by Student t test; *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. (C) Hematoxylin and eosin staining of ear sections. (Scale bar, 40 µm.) (D) Immunohistochemistry analyses of ear sections using a TSLP-specific antibody (stained in red). (Scale bar, 20 µm.) (E) The qRT-PCR for RNA transcripts of proinflammatory genes in ears of mouse treated, as indicated. Th1-, Th2-, and Th17-specific proinflammatory interleukins are highlighted. The statistical significance compared with the TPA treatment was calculated by Student t test; *P < 0.05; **P < 0.01; ***P < 0.001. (F) The qRT-PCR for transcripts of proinflammatory genes in mouse ears treated as indicated. The statistical significance compared with the Aldara treatment was calculated by Student t test; *P < 0.05; **P < 0.01; ***P < 0.001. All data are represented as mean ± SEM of at least three independent experiments with at least three mice per treatment.