Fig. 2.
CpdX, CpdX(eA), CpdX-D3, and CpdX-D3(eA), but not CpdX(eB) or CpdX-D3(eB), are as efficient as Dex at decreasing an HDM-induced asthma-like lung allergic inflammation. (A) Experimental protocol for generating an HDM-induced asthma-like lung allergic inflammation. (B) Cell counting from the fluid of BAL. (C) The qRT-PCR for RNA transcripts of proinflammatory genes in lungs of mice treated as indicated. Th2-specific proinflammatory interleukins are highlighted. Data are represented as mean ± SEM of at least three independent experiments with at least four mice per treatment. The statistical significance, compared with the HDM treatment (for B and C), was calculated by Student t test; *P < 0.05; **P < 0.01; ***P < 0.001. (D) Hematoxylin and eosin staining of lung sections. Peribronchiolar (B) and perivascular (V) regions are indicated. (Scale bar, 40 µm.) (E) AHR determined using the FlexVent system after exposure to 50 mg/mL methacholine. Data are represented as mean ± SEM of at least two independent experiments with at least eight mice per treatment. The statistical significance compared with the HDM treatment was calculated through two-way ANOVA followed by Bonferroni multiple comparisons; **P < 0.01; ***P < 0.001; ****P < 0.0001.