Fig. 2.

Unlike Dex, a 3-mo mouse treatment with CpdX, CpdX-D3, or their enantiomers does not affect bone formation. Five cortical bone parameters were measured by micro-CT: (A) bone volume/total volume (%); (B) cortical thickness (mm); (C) bone area (mm²), (D) marrow area (mm²) and (E) total area (mm²); the data correspond to the mean ± SEM for, at least, six mice per treatment. The statistical significance compared with vehicle treatment was calculated through a one-way ANOVA test followed by Dunnett’s multiple comparison test (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; (****) P < 0.0001; (ns): not significant. (F) Q-RT-PCR for transcripts of the WNT16 gene in tibia from mice treated as indicated. Data are represented as mean ± SEM with, at least, six mice per treatment. The statistical significance was calculated by Student t test; (***) P < 0.001.