Table 1.

Enzymatic activity of GST P1-1 in cells overexpressing GST P1-1 WT and C47S, C101S, and C47S/C101S mutant enzymes

Samples	Specific activity, U/mg	Fold increase vs. SH-SY5Y
SH-SY5Y	0.195 ± 0.09	—
SH-SY5Y pTarget GST P1-1	1.865 ± 0.29	9.6
SH-SY5Y pTarget GST P1-1 C47S	0.476 ± 0.04	2.4
SH-SY5Y pTarget GST P1-1 C101S	0.990 ± 0.07	5.1
SH-SY5Y pTarget GST P1-1 C47S/C101S	0.430 ± 0.06	2.2

The enzymatic activity was determined as reported in the Materials and Methods section. The fold increases in the enzymatic activity of the transfected vs. untransfected cells are reported. Values are expressed as means ± SD from 3 independent experiments.