Table 2.

Observed mass peaks of GST P1-1 and its mutant forms incubated with cis-DDP

Enzyme	Peak	Molecular mass, Da	Assignment	Relative abundance, %
GST P1-1	A	23,216	GST P1-1 monomer	50
	B	23,347	GST P1-1* monomer	100
	C	46,433	GST P1-1 dimer	4
	D	23,216	GST P1-1 monomer	100
	E	46,860	GST P1-1 dimer + 2×[Pt(NH3)]	74
	F	47,093	GST P1-1 dimer + 2×[Pt(NH3)] + [Pt(NH3)2]	82
	G	47,429	GST P1-1* dimer + 3×[Pt(NH3)Cl]	91
C47S	A	23,199	C47S monomer	98
	B	23,330	C47S* monomer	89
	C	46,398	C47S dimer	5
	D	23,753	C47S* monomer + 2×[Pt(NH3)]	100
	E	46,824	C47S dimer + 2×[Pt(NH3)]	54
	F	47,164	C47S dimer + 2×[PtCl2] + [Pt(NH3)2]	24
C101S	A	23,199	C101S monomer	36
	B	23,330	C101S* monomer	97
	C	46,398	C101S dimer	5
	D	23,199	C101S monomer	30
	E	23,235.5	C101S monomer + MeOH	100
	F	23,524	C101S* monomer + Pt	26
	G	23,541	C101S* monomer + [Pt(NH3)]	16
	H	23,734	C101S monomer + 2×[PtCl2]	28
	I	23,752	C101S* monomer + 2×[Pt(NH3)]	74
	L	46,464.5	C101S dimer + 2×MeOH	18

The relative abundance (%) of each peak is obtained after normalization to the tallest peak present in each spectra.

^*Enzymes characterized by the presence of the N-terminal methionine residue.