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. 2019 Jul 8;10:826. doi: 10.3389/fphys.2019.00826

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Copyright © 2019 Zhang, Wang, Yanni, Qureshi, Logantha, Kassab, Boyett, Gardiner, Sun, Howarth and Dobrzynski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot of HCN4, Na_v1.5, Ca_v1.3 and Ca_v3.1 and in STZ-induced diabetic rats vs. control rats. (A–D) Mean relative abundance (normalized to β-actin) of HCN4, Na_v1.5, Ca_v1.3, and Ca_v3.1 in the LA (left atrium), SAN (sinoatrial node), AVJ (atrioventricular junction), LPF (left Purkinje fibers) and RV (right ventricle). (E) Western blot of SDS polyacrylamide gel electrophoresis of homogenized tissue samples obtained from STZ-induced type I diabetic hearts and controls labeled for HCN4, Na_v1.5 Ca_v1.3, and Ca_v3.1 proteins. ^*Significantly different from control. Data are mean ± SEM (n = 5 for each group); P < 0.05.