Fig. 2.

—Gene expression pattern according to the location along the Z chromosome, and the developmental stages. The female-to-male expression ratio (F:M) is represented for the three Z-specific regions defined in the version 5.2 of the genome (Z1 in light beige, Z2 in orange, and Z3 in light beige) and the PAR (in dark beige) for cercariae (A), schistosomula (C), and immature worms (E). For each stage, gene expression pattern for female (in pink) and male (in blue) is shown along the Z by sliding window of 50 genes (B, D, F). The thick black line represents the female-to-male expression ratio by sliding window of 50 genes. Only genes with expression RPKM > 1 and a sex-bias fold change <2 are shown. Asterisks show the level of significance of Z-to-PAR comparisons (Wilcoxon rank sum test with continuity correction): ***P value < 0.0001, N.S. = nonsignificant differences. Z1-to-PAR ratio values are 0.76, 0.72, and 0.64; Z2-to-PAR ratio values are 0.88, 0.71, and 0.62; Z3-to-PAR ratio values are 0.81, 0.74, and 0.62, for cercariae, schistosomula, and immature worms, respectively. Other ratios are shown in Supplementary Table 2, Supplementary Material online.