Skip to main content
. Author manuscript; available in PMC: 2019 Dec 24.
Published in final edited form as: Nat Struct Mol Biol. 2019 Jun 24;26(7):613–618. doi: 10.1038/s41594-019-0255-5

Fig. 4. SRAP can accommodate dsDNA 3′ to the AP site.

Fig. 4.

a, Model of YedK DPC with a 3′ junction at AP site. The modeled complementary DNA strand is pink. The wedge domain blocking dsDNA access 5′ to the AP site is blue. b, Wedge-DNA interactions 5′ to the AP site. c, Sequence conservation of the DNA shelf that presumably stabilizes dsDNA 3′ to the AP site. d, EMSA showing binding of human HMCES SRAP domain to the indicated DNA ligands. The plot shows mean ± S.E.M from n = 3 independent measurements. Uncropped gel image is shown in Supplementary Data Set 1. e, Percent of the indicated DNA substrates crosslinked to human HMCES SRAP domain (mean ± S.D., n=3 independent measurements). Source data for d,e are available online.