Table 1.
Data collection and refinement statistics
| YedK/AP-DNA Covalent DPC (6NUA) | YedK/C3spacer-DNA Non-covalent complex (6NUH) | |
|---|---|---|
| Data collection | ||
| Space group | P21 | P21 |
| Cell dimensions | ||
| a, b, c (Å) | 61.26, 41.89, 81.42 | 47.54, 44.13, 55.09 |
| α, β, γ (°) | 90.00, 95.79, 90.00 | 90.00, 102.34, 90.00 |
| Resolution (Å) | 50.00–1.64 (1.67–1.64)a | 100.00–1.60 (1.66–1.60) |
| Rsym | 0.098 (0.500) | 0.075 (0.397) |
| Rmeas | 0.110 (0.595) | 0.086 (0.455) |
| I/σ(I) | 14.8 (1.9) | 21.3 (2.6) |
| CC1/2 | 0.989 (0.823) | 0.990 (0.869) |
| Completeness (%) | 97.4 (95.2) | 98.5 (91.1) |
| Redundancy | 4.4 (2.9) | 4.1 (4.0) |
| Refinement | ||
| Resolution (Å) | 40.50–1.64 (1.67–1.64) | 39.60–1.59 (1.65–1.59) |
| No. reflections | 49,681 (2,331) | 29,612 (2,391) |
| Rwork / Rfree | 0.770 (0.873) | 0.803 (0.829) |
| No. atoms | ||
| Protein | 3,627 | 1,802 |
| DNA | 268 | 131 |
| Bis-Tris | - | 14 |
| Water | 280 | 210 |
| B factors | ||
| Protein | 26.0 | 17.1 |
| DNA | 28.7 | 64.3 |
| Bis-Tris | - | 38.3 |
| Water | 29.3 | 24.4 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.010 | 0.008 |
| Bond angles (°) | 1.035 | 0.959 |
Data for each structure were generated from a single crystal.
a
Values in parentheses are for highest-resolution shell.