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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cell Rep. 2019 Jun 11;27(11):3107–3116.e3. doi: 10.1016/j.celrep.2019.05.056

Figure 4. LRIT3 Expression in Rods Restores Normal ON RGC Function in Lrit3−/− Retinas.

Figure 4.

(A) Example of raster plots and peri-stimulus time histograms (PSTH) of each cell type as indicated, recorded on the MEA (multi-electrode array).

(B) Frequency distribution of time to peak (TTP) for the ON responses in control (i), Lrit3−/− (ii), and rAAV RHO::Lrit3-treated Lrit3−/− (iii) retinas.

(C) Percentage of each cell type recorded under scotopic (i) and photopic (ii) conditions. dON and dON/OFF cells are omitted because of their small numbers, but see Table S1 for numbers. All groups were analyzed using chi-square tests, and when significant (p < 0.05) two-way post hoc tests were done (chi-square) with Bonferroni adjustment of the p values for multiple testing (*padj < 0.025).

(D and E) Example PSTH’s for a cell from control (Di) and rAAV RHO::Lrit3 retinas (Ei) in Ringer’s solution, in Ringer’s plus 5 mM L-AP4, and after L-AP4 washout. (Dii) The peak responses for individual cells (gray lines) and mean ± SEM (red lines) of control (Dii) and rAAV RHO::Lrit3-treated Lrit3−/− (Eii) retinas are shown. The response amplitudes in (D) and (E) in L-AP4 was significantly decreased (*padj < 0.0001, repeated-measures ANOVA, Bonferroni corrected for both datasets) and after washout was not significant (ns; padj > 0.4). padj, adjusted p value.