Skip to main content
. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Cell Rep. 2019 Jun 11;27(11):3107–3116.e3. doi: 10.1016/j.celrep.2019.05.056

Figure 5. LRIT3 Expression in Rods of Adult Lrit3−/− Mice Restores RBC Function.

Figure 5.

(A) Whole mount of retinas from P35 rAAV RHO::Lrit3-treated Lrit3−/− mice stained for LRIT3 (magenta) and TRPM1 (green) analyzed at P70. A sample of the LRIT3 puncta is highlighted (dashed circles), showing that they co-localize with TRPM1. Every LRIT3 puncta co-localizes with TRPM1 puncta. Scale bar, 2 μm.

(B) Quantification of the percentage of rod synapses expressing LRIT3 and TRPM1 in WT (black symbol), rAAV RHO::Lrit3-injected animals at P5 (green symbol) or P35 (blue symbol), and Lrit3−/− retinas (red symbol). Note that the P5 data are replicated from Figure 1.

(C and D) Scotopic (C) and photopic (D) electroretinograms at different stimulus intensities for one control eye (black), an age-matched Lrit3−/− eye (red), and an rAAV RHO::Lrit3-treated Lrit3−/− P35 mouse eye (blue).

(E) Scotopic responses to a −3.6 log cd s/m2 stimulus for a control rAAV RHO::GFP (black), a Lrit3−/− (red), and five rAAV RHO::Lrit3-injected P35 Lrit3−/− (blue) mouse eyes.

(F) Response amplitudes from the five animals in (E) (blue triangles) and WT (black circles) to the −3.6 log cd s/m2 stimuli. The red line indicates no b-wave (zero amplitude) in the Lrit3−/− animals (mean ± SD are indicated. The WT data are from Figure 3C.

Statistics: (F) rAAV RHO::Lrit3-injected Lrit3−/− eyes show significantly greater b-waves than Lrit3−/− groups (b-wave amplitude = 0), *p < 0.01 (single-sample t test). rAAV RHO::Lrit3-injected Lrit3−/− eyes show significantly smaller b-waves than WT eyes, **p < 0.001 (two-sided t test).