KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Guinea pig anti-LRIT3 | Ronald Gregg, Hasan et. al. (2018) | N/A |
Rabbit anti-TRPMI | Ronald Gregg; Hasan et. al. (2018) | N/A |
Sheep anti-mGluR6 | Kiril Martemyanov; Cao et. al. 2011 | N/A |
Donkey anti-sheep IgG-AlexaFluor 488 | Life Technologies | Cat # A111015 |
Donkey anti-rabbit IgG-AlexaFluor 647 | Life Technologies | Cat # A31573 |
Donkey anti-guinea pig IgG-Cy3 | Millipore | Cat # AP193C |
Donkey anti-rabbit IgG-AlexaFluor 488 | Life Technologies | Cat# A21206 |
Bacterial and Virus Strains | ||
pAAV-RHO::Lrit3 | This paper | Addgene; Cat # 126511 |
rAAV2/2[MAX] capsid | Reid et al., 2017 | N/A |
Critical Commercial Assays | ||
In-Fusion® HD Cloning Plus | Takara | Cat # 638910 |
Experimental Models: Organisms/Strains | ||
Mouse: Lrit3em1Rgg | Ronald Gregg | MGI: 5645054 |
C57BL/6J | Jackson Labs | Stock # 000664; RRID:IMSR_JAX:000664 |
Oligonucleotides | ||
Lrit3 KO genotyping forward | CTTTAAACGGAGTCTCGAAGC | N/A |
Lrit3 KO genotyping reverse | CTGACCGCCTCGTTTGGCAC | N/A |
Software and Algorithms | ||
Prism 8.1.0 (325) | Graphpad Software | RRID:SCR_002798 |
MC_Rack 4.6.2 | Multi Channel System | RRID:SCR_014955 |
Offline Sorter 3.3.5 | Plexon Inc | RRID:SCR_000012 |
NeuroExplorer 5.103 | Nex Technologies | RRID:SCR_001818 |
Fluoview | Olympus | RRID:SCR_017015 |
Other | ||
RNAScope® in situ hybridization | Grm6; Genbank:NM_173372.2 | N/A |
RNAScope® in situ hybridization | Mm-Pol2ra ; positive control | Cat # 312471 |
RNAScope® in situ hybridization | DapB ; negative control | Cat # 310043 |