Figure 7. Inter-organ integration of RYGB-induced metabolic responses 9 days and 9 weeks after surgery in mice.

A–B. Principal component analysis (PCA) of log₂ fold change in metabolic gene expression across the 6 studied tissues in mice. Orange circles denote genes with a Z-score smaller than −2.5 or greater than 2.5 (see Quantification and Statistical Analysis).

C–E. Mean log₂ fold change in gene expression levels across tissues in: (C) genes associated with transcription factors that were found to play a role in the coordination of the transcriptional response based on the PCA, as shown in panels A–B. See also supplementary file Data S1. RYGB-treated vs. WMS mice; duod: duodenum, jejun: jejunum, (D) Il33 (red) and Ghr (blue), (E) components of growth hormone receptor pathway.

F. Immunohistochemical staining for GHR in liver sections. Scale bars: 50μm. Slides were counterstained with hematoxylin.

G. Pearson correlation matrix (mean log₂ fold change in differentially expressed genes) across tissues and time-points in mice (RYGB-treated vs. WMS). Gray denotes a correlation of 1 to itself. Dashed gray line corresponds to correlation of a tissue to itself 9 days and 9 weeks after surgery.

* p<0.05, **p<0.01, both for RYGB vs. WMS independent samples t-test.