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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Science. 2018 Feb 15;360(6387):436–439. doi: 10.1126/science.aar6245

Fig. 1. Cas12a target recognition activates non-specific single-stranded DNA cleavage.

Fig. 1.

(A) Cas12a-crRNA complex binds a dsDNA substrate and generates a 5’ overhang staggered cut using a single RuvC nuclease. (B, C) Representative M13 ssDNA cleavage timecourses with purified LbCas12a (left) and SpCas9 (right) complexed with a (B) guide RNA complementary to M13 phage or (C) a guide RNA and complementary ssDNA activator with no sequence homology to M13 phage.