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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Methods Mol Biol. 2009;511:85–105. doi: 10.1007/978-1-59745-447-6_4

Fig. 14.

Fig. 14.

Schematic of a four-plex SearchLight® Array and image of a 16-plex SearchLight® Array. Each antibody spot captures a specific cytokine, chemokine, or other biomarker that is then detected with a biotinylated antibody cocktail followed by addition of streptavidin-horseradish peroxidase (SA-HRP) and SuperSignal® ELISA Chemiluminescent Substrate. The light produced from the HRP-catalyzed oxidation of the substrate is measured by imaging the plate with a cooled CCD camera. Standard curves are generated using a mixture of the recombinant array proteins. Protein concentrations in a sample are then quantified by comparing the intensity of the spots to the corresponding standard curve. SuperSignal® Technology is protected by U.S. patent # 6,432,662.