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. Author manuscript; available in PMC: 2019 Nov 8.
Published in final edited form as: Nat Med. 2019 May 8;25(5):850–860. doi: 10.1038/s41591-019-0404-8

Fig.4 ∣. Revealing amino acid metabolism auxotrophs by pooled cancer cell line screens.

Fig.4 ∣

a, Scatter plot comparing ASNS DNA methylation levels with ASNS mRNA levels in all cell lines. b, Schematic summarizing the workflow of pooled cancer cell line screens. c-e, Waterfall plots showing the fold changes of pooled CCLE lines (n=554, median of 3 independent cell culture replicates) cultured in RPMI media containing 0.1 μM asparagine (c), 0.1 μM arginine + 1 mM L-citrulline (precursor required for arginine synthesis) (d), 0.1 μM glutamine (e) for 6 days (normalized to control). For c-e, the p-values were calculated based on the significance test of Pearson correlations (two-sided).