Figure 2. Effect of HSCT on lymphocyte phenotype and differentiation in DOCK8-deficient patients.

PBMCs from healthy donors (n = 22–24), untransplanted DOCK8-deficient patients (n = 7–9), or DOCK8-deficient patients following HSCT (DOCK8 pHSCT) (n = 18–20) were labeled with mAbs against CD3, CD4, CD8, CD20, CD56, CD45RA, CCR7, CD10, CD27, IgM, IgD, TCRαβ, TCRγδ, TCRVα24, TCRVβ11, CD161, and TCR Vα7.2. Proportions of (A) CD3⁺ cells, CD4⁺ T cells (CD3⁺CD4⁺), CD8⁺ T cells (CD3⁺CD8⁺), B cells (CD20⁺); (B) CD8⁺ naive (CD45RA⁺CCR7⁺), central memory (T_CM; CD45RA^–CCR7⁺), effector memory (T_EM; CD45RA^–CCR7^–), and CD45RA⁺ revertant memory (T_EMRA; CD45RA⁺CCR7^–) cells; (C) CD4⁺ naive, T_CM, and T_EM cell subsets; (D) αβ and γδ TCR⁺ T cells; (E) MAIT cells (CD3⁺TCRVα7.2⁺CD161⁺); (F) NK cells (CD3^–CD56⁺); (G) NKT cells (CD3⁺TCRVα24⁺ Vβ11⁺); (H) transitional (Trans; CD20⁺CD10⁺CD27^–), naive (CD20⁺CD10^–CD27^–), and memory (CD20⁺CD10^–CD27⁺) B cell subsets; and (I) Ig class-switched memory (CD20⁺CD27⁺ IgD^–IgM^–) B cells were then determined by flow cytometric analysis. Contour plots represent 1 representative normal donor and 1 DOCK8-deficient patient before and after HSCT. Data are mean ± SEM. Statistical analysis was performed with GraphPad Prism using unpaired t test with Welch’s correction; *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.