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. 2019 Jun 6;4(11):e127527. doi: 10.1172/jci.insight.127527

Figure 6. B cell functional defects due to DOCK8 deficiency are improved following HSCT.

Figure 6

(A) Naive B cells were sort-purified from healthy donors and DOCK8-deficient patients (n = 7) and then cultured with CD40L/IL-21, CD40L/CpG, CpG/BCR agonist (Staphylococcus aureus Cowan I), or CD40L/CpG/BCR. After 11 days, culture supernatants were harvested and levels of secreted IgM, IgG, and IgA then determined. Data represent mean ± SEM. (B–D) Naive B cells were sort-purified from healthy donors (n = 18–23), untransplanted DOCK8-deficient patients (n = 5–7), and DOCK8-deficient patients following HSCT (DOCK8 pHSCT) (n = 12–15); labeled with CFSE; and then cultured with combinations of CD40L, IL-21, CpG, and BCR stimulus for 5 days. After this time, cells and culture supernatants were harvested. (B) Cell number was determined using Calibrite beads. (C) Frequency of cells in each division was determined by CFSE dilution. Histogram plots show CFSE dilution from 1 representative healthy donor and 1 paired DOCK8-deficient patient before and after HSCT. (D) Secretion of IgM, IgG, and IgA was determined by ELISAs. Data in each graph represent mean ± SEM. Statistical analysis was performed with Prism using unpaired t test with Welch’s correction; *P < 0.05, **P < 0.01, ****P < 0.001.