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. 2019 Jun 20;4(12):e126124. doi: 10.1172/jci.insight.126124

Figure 2. IFN-γ induces kidney APOL1 expression in APOL1 G0 and G1 mice, but proteinuria only in G1 mice.

Figure 2

Female APOL1 G0– and G1–transgenic and WT littermate mice (n = 3–4) were challenged with a single dose of IFN-γ (1.125 × 107 U/kg) or vehicle (PBS). (A) Urine was collected 24, 48, and 72 hours after IFN-γ challenge, and urinary albumin was measured by ELISA and normalized to urine creatinine. (B) Kidney Irf1 expression was measured by qRT-PCR 24 and 72 hours after IFN-γ challenge and normalized to Cyp expression. (C) Kidney and (D) liver APOL1 expression were measured by qRT-PCR 24 and 72 hours after IFN-γ challenge and normalized to Cyp expression. (E) Liver Irf1 expression was measured by qRT-PCR 24 and 72 hours after IFN-γ challenge and normalized to Cyp expression. All data are presented as mean ± SD. Two-way ANOVA with Tukey’s multiple comparisons test, ***P < 0.001.