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. 2019 Jun 20;4(12):e125494. doi: 10.1172/jci.insight.125494

Figure 2. BeO exposure resulted in the formation of ectopic lymphoid aggregates (ELAs) and granulomatous inflammation in HLA-DP2–Tg mice.

Figure 2

HLA-DP2–Tg mice were exposed to BeO as described in Methods, lungs were harvested on day 21, and serial sections were analyzed by H&E and reticulin staining (A–D) or were analyzed by immunohistochemical staining for CD4 and B220 (E and F). BeO exposure of HLA-DP2–Tg mice induced ELAs (A and B) and granuloma formation (C and D) in the lungs of HLA-DP2–Tg mice (H&E stained in A and B; reticulin stained in C and D; original magnification, ×40). Yellow arrows in B and D depict the distribution of type IV collagen within the aggregates and granulomas, respectively. The yellow arrow in C depicts a multinucleated giant cell. (E) Immunohistochemical staining of CD4+ cells and (F) B220+ cells infiltrating the lungs of BeO-exposed HLA-DP2–Tg mice. Original magnification (E and F), ×2 (left) and ×20 (right). Histology images are representative of 5 to 6 animals per group from 3 independent experiments. Scale bars: 20 μm. The average size (G) and number (H) of ELAs in the lungs of BeO-exposed HLA-DP2–Tg mice are shown. Statistical significance was determined using a Student’s t test (2 tailed), and the solid lines and error bars depict the mean ± SEM. A P value of <0.05 was considered statistically significant.