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. 2019 Jun 20;4(12):e125494. doi: 10.1172/jci.insight.125494

Figure 7. BeO exposure enhanced acute lung injury in HLA-DP2–Tg mice deficient in B cells.

Figure 7

(A and E) Low-magnification view of H&E-stained lung tissue collected from BeO-exposed HLA-DP2–Tg mice treated with isotype control (A) or anti-CD20 (E) mAb is shown. (B and F) Higher-magnification view of A and B is shown. Dark-field microscopic images show BeO particle distribution (white nodules) in the lungs of BeO-exposed mice treated with either isotype control (C) or anti-CD20 (G) mAb. High-magnification view of alveolar tissue in BeO-exposed HLA-DP2–Tg mice treated with isotype control (D) or anti-CD20 (H) mAb is shown. Scale bars: 20 μm. (I) Quantitative analysis of BeO particles within the granuloma and alveolar space. Concentrations of total protein (J), albumin (K), and podoplanin (TIA) (L) measured by ELISA in BALF from isotype control or anti-CD20 mAb–treated BeO-exposed HLA-DP2–Tg mice is shown. Cumulative data from 2 independent experiments having 3 to 5 mice per group are shown, and the mean ± SEM concentration (pg/ml) is shown as a solid line with error bars. Statistical significance was determined using a Student’s t test (2 tailed) (I) or 2-way ANOVA (J–L), and a P value of <0.05 was considered statistically significant.