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. 2019 Jun 20;4(12):e121291. doi: 10.1172/jci.insight.121291

Figure 7. PARP-1 promotes NF-κB nuclear translocation and DNA binding in microphages.

Figure 7

Peritoneal macrophages from WT or Parp-1–/– mice were infected with VV (MOI = 0.5) for 1 hour or left uninfected as a control (Ctrl). (A) After staining with anti–NF-κB-p65 (green) and nuclear (blue) staining, representative confocal microscopy images are shown. Original magnification, ×200. (B) NF-κB containing nuclear extract was measured for the ability to bind to a specific double-stranded DNA (dsDNA) sequence containing the NF-κB-p65 response element by a colorimetric assay. Data represent mean OD450 readouts ± SD (n = 3). **P < 0.01, 2-tailed Student’s t test.