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. 2019 Jun 6;4(11):e126910. doi: 10.1172/jci.insight.126910

Figure 6. SGK1 phosphorylation and ENaC current are regulated by basolateral [K+].

Figure 6

(A) Effect on amiloride-sensitive current of changing apical (left panel) versus basolateral (right panel) [K+] from 1 mM to 5 mM in mpkCCD cells. Data are presented as mean ± SEM. *P < 0.05 by 2-tailed Student’s t test. NS, not significant. (B) Effect on endogenous p-SGK1 S422 level of changing apical versus basolateral [K+] from 1 mM to 5 mM. Upper panel: Western blot images showing blots stained with anti–phospho-SGK1 S422, and total SGK1 as labeled. Lower panels: Quantitation of phospho-SGK1/total SGK1 in response to changes in apical (left panel) versus basolateral (right panel) [K+] (as described in Methods). Data are presented as mean ± SEM. *P < 0.05 by 2-tailed Student’s t test. Raising basolateral but not apical [K+] stimulates SGK1 S422 phosphorylation and Na+ current.