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. 2019 Jun 6;4(11):e128241. doi: 10.1172/jci.insight.128241

Figure 7. Rejection of BALB/c→B6 lung grafts treated with intratracheal eotaxin 1, 2, and IL-5.

Figure 7

BALB/c lungs were transplanted into B6 recipients, which were treated with an intratracheal chemokine and cytokine cocktail of eotaxin 1, 2, and IL-5 immediately after engraftment and 1 day after transplantation. Lung grafts were evaluated flow cytometrically and histologically after 7 days for rejection (A), total eosinophil content (B), and CD8+ T cell differentiation (C). Histologic analysis for ISHLT grade of rejection was performed on slides stained with hematoxylin and eosin (H&E), and eosinophil peroxidase immunohistochemistry was performed to evaluate eosinophil orientation in the tissue (eosinophils stained red). Scale bars: 100 μm. All statistics performed by Mann-Whitney U test. *P < 0.05.