Skip to main content
. 2019 Jul 11;4(13):e99052. doi: 10.1172/jci.insight.99052

Figure 1. Incorporating miR142BSs into rAAV1 expression cassettes decreases transgene expression in APCs.

Figure 1

(A) Schematics of rAAV OVA expression vectors. ITRs, inverted terminal repeats; CB, CMV enhancer/chicken β-actin promoter; pA, polyA. (B) Endogenous miR-142-3p mRNA expression levels in cultured JAWS II (DCs), RAW264.7 (macrophages), or C2C12 (myoblasts) cells as quantified by reverse transcription quantitative PCR (RT-qPCR) (n = 2). (C) JAWS II, RAW264.7, or C2C12 cells were transfected with OVA or OVA.miR142BS plasmids. Seventy-two hours after transfection, supernatants were collected for OVA quantification by ELISA. Bar graphs represent mean ± SD (n = 3). **P < 0.01, unpaired t test.